The availability of a high-quality, chromosome-level reference genome accelerates breeding programs. A reference genome assembled to large scaffolds or even full-length chromosomes reveals the genome architecture as well as locations of specific genes and markers. Insight into gene order allows QTL analyses to be performed without the need of a physical map. Additionally, questions involving structural variant detection can be answered, facilitating prediction of recombination frequencies along the genome. A high-quality reference genome is also a valuable addition to breeding programs involving commercial varieties that lack specific traits that are available in wild germplasm.
Advanced statistical models are needed to increase the power to discover (new) associations between genetic and phenotypic variation.