Understanding both, phenotypic and genetic, variation among plants, starts with an adequate experimental design to provide sensible phenotypic and genotypic information. To set-up an experimental design, first the main question has to be defined, whereupon recommendations regarding e.g. size of the multi-parental population, number crossings, traits of interest with measuring protocols, and environments (multi-environmental trails) can be made. Genetwister offers assistance in the design of experiments, with awareness of breeding program’s practical considerations.
Advanced statistical models are needed to increase the power to discover (new) associations between genetic and phenotypic variation.